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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using RNF167 Polyclonal antibody (24618-1-AP)

    WB analysis of SH-SY5Y using 24618-1-AP (same clone as 24618-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RNF167 Polyclonal antibody (24618-1-AP)

    WB analysis of mouse testis using 24618-1-AP (same clone as 24618-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using RNF167 Polyclonal antibody (24618-1-AP)

    WB analysis of mouse brain using 24618-1-AP (same clone as 24618-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RNF167 Polyclonal antibody, PBS Only

RNF167 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24618-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24618-1-PBS targets RNF167 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF167 fusion protein Ag18383 相同性解析による交差性が予測される生物種
Full Name ring finger protein 167
Calculated molecular weight 350 aa, 38 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC010139
Gene Symbol RNF167
Gene ID (NCBI) 26001
RRIDAB_2879642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Y7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).