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RNF168 Polyclonal antibody

RNF168 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 21393-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, CoIP, ELISA

RING-type E3 ubiquitin transferase RNF168, RING finger protein 168, hRNF168, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF168

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human brain tissue, Jurkat cells, Transfected HEK-293 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21393-1-AP targets RNF168 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF168 fusion protein Ag16055 相同性解析による交差性が予測される生物種
Full Name ring finger protein 168
Calculated molecular weight 571 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC033791
Gene Symbol RNF168
Gene ID (NCBI) 165918
RRIDAB_10733883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF168 antibody 21393-1-APDownload protocol
IF protocol for RNF168 antibody 21393-1-APDownload protocol
IP protocol for RNF168 antibody 21393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

Authors - Kaiwen Bao
humanIP

Nat Commun

Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.

Authors - Qinglei Hang
human

Nucleic Acids Res

GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.

Authors - Xiaopeng Lu
humanWB

Theranostics

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

Authors - Qi Zhang
humanWB,IF

J Cell Biol

DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response

Authors - Tessa Arends
humanWB

EMBO Rep

An LTR retrotransposon-derived lncRNA interacts with RNF169 to promote homologous recombination.

Authors - Bing Deng
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