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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF181 Polyclonal antibody, PBS Only

RNF181 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20408-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RNF181 Polyclonal antibody (20408-1-AP)

    WB analysis of Jurkat using 20408-1-AP (same clone as 20408-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using RNF181 Polyclonal antibody (20408-1-AP)

    WB analysis of L02 using 20408-1-AP (same clone as 20408-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using RNF181 Polyclonal antibody (20408-1-AP)

    IP experiment of Jurkat using 20408-1-AP (same clone as 20408-1-PBS)

    IP result of anti-RNF181 (IP:20408-1-AP, 3ug; Detection:20408-1-AP 1:500) with Jurkat cells lysate 1200ug. This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RNF181 Polyclonal antibody (20408-1-AP)

    IHC staining of human kidney using 20408-1-AP (same clone as 20408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RNF181 Polyclonal antibody (20408-1-AP)

    IHC staining of human kidney using 20408-1-AP (same clone as 20408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using RNF181 Polyclonal antibody (20408-1-AP)

    IHC staining of human heart using 20408-1-AP (same clone as 20408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using RNF181 Polyclonal antibody (20408-1-AP)

    IHC staining of human heart using 20408-1-AP (same clone as 20408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF181 Polyclonal antibody (20408-1-AP)

    IF Staining of HepG2 using 20408-1-AP (same clone as 20408-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF181 antibody (20408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20408-1-PBS targets RNF181 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF181 fusion protein Ag14233 相同性解析による交差性が予測される生物種
Full Name ring finger protein 181
Calculated molecular weight 153 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002803
Gene symbol RNF181
Gene ID (NCBI) 51255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Western blot analysis detected RNF181 protein in human platelets as a 36-kD dimer and an 18-kD monomer.