Featured Product
KD/KO Validated

RNF2 Polyclonal antibody

Name: RNF2 Polyclonal antibody
Brand: Proteintech
SKU: 16031-1-AP

RNF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16031-1-AP

Synonyms

HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of K-562 using 16031-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16031-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16031-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 16031-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 16031-1-AP
IP result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug.
IF Staining of HeLa using 16031-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16031-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, human brain tissue, NIH/3T3 cells, U-937 cells
Positive IP detected in	K-562 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 13 publications below
IHC	See 5 publications below
IF	See 3 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

16031-1-AP targets RNF2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RNF2 fusion protein Ag8900 相同性解析による交差性が予測される生物種
Full Name	ring finger protein 2
Calculated molecular weight	336 aa, 38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC012583
Gene symbol	RNF2
Gene ID (NCBI)	6045
RRID	AB_2180459
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF2 antibody 16031-1-AP	Download protocol
IF protocol for RNF2 antibody 16031-1-AP	Download protocol
IP protocol for RNF2 antibody 16031-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Coordinated regulation of polycomb group complexes through microRNAs in cancer. Authors - Qi Cao KD Validated View Article
human	IHC	Cancer Cell The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression. Authors - Wenjing Su KD Validated View Article
		Dev Cell Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells Authors - Wenxiu Dai View Article
human	WB,IF	J Cell Biol DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response Authors - Tessa Arends KD Validated View Article
human	WB,IHC	FEBS J Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy Authors - Yan Wei View Article
human	WB, IF	Front Cell Dev Biol RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells. Authors - Qi Yan KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF2 Polyclonal antibody

RNF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of K-562 using 16031-1-AP

WB analysis of NIH/3T3 using 16031-1-AP

WB analysis of human brain using 16031-1-AP

WB analysis of U-937 using 16031-1-AP

IP experiment of K-562 using 16031-1-AP

IF Staining of HeLa using 16031-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Coordinated regulation of polycomb group complexes through microRNAs in cancer.

The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy

RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells.