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  • Featured Product
  • KD/KO Validated

RNF20 Polyclonal antibody, PBS Only

RNF20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21625-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF20 Polyclonal antibody (21625-1-AP)

    WB analysis using 21625-1-AP (same clone as 21625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RNF20 Polyclonal antibody (21625-1-AP)

    WB analysis using 21625-1-AP (same clone as 21625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RNF20 Polyclonal antibody (21625-1-AP)

    WB analysis of MCF-7 using 21625-1-AP (same clone as 21625-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using RNF20 Polyclonal antibody (21625-1-AP)

    WB analysis of mouse liver using 21625-1-AP (same clone as 21625-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RNF20 Polyclonal antibody (21625-1-AP)

    IP experiment of HeLa using 21625-1-AP (same clone as 21625-1-PBS)

    IP result of anti-RNF20 (IP:21625-1-AP, 3ug; Detection:21625-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RNF20 Polyclonal antibody (21625-1-AP)

    IP experiment of HeLa using 21625-1-AP (same clone as 21625-1-PBS)

    IP result of anti-RNF20 (IP:21625-1-AP, 3ug; Detection:21625-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RNF20 Polyclonal antibody (21625-1-AP)

    IHC staining of mouse kidney using 21625-1-AP (same clone as 21625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21625-1-AP (RNF20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RNF20 Polyclonal antibody (21625-1-AP)

    IHC staining of mouse kidney using 21625-1-AP (same clone as 21625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21625-1-AP (RNF20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF20 Polyclonal antibody (21625-1-AP)

    IF Staining of HepG2 using 21625-1-AP (same clone as 21625-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF20 antibody (21625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNF20 Polyclonal antibody (21625-1-AP)

    IF Staining of HeLa using 21625-1-AP (same clone as 21625-1-PBS)

    Immunofluorescent analysis of Hela cells, using RNF20 antibody 21625-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21625-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21625-1-PBS targets RNF20 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF20 fusion protein Ag16066 相同性解析による交差性が予測される生物種
Full Name ring finger protein 20
Calculated molecular weight 975 aa, 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberBC110585
Gene symbol RNF20
Gene ID (NCBI) 56254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNF20, also named as E3 ubiquitin-protein ligase BRE1A, is a 975 amino acid protein, which contains 1 RING-type zinc finger and belongs to the BRE1 family. RNF20 is a component of the RNF20/40 complex and localizes in the nucleus. RNF20 is a component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B. RNF20 is required for transcriptional activation of Hox genes and recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions.