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Validation Data Gallery

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  • Western Blot (WB) analysis of SW480 cells using RNF216 Recombinant antibody (83658-2-RR)

    WB analysis of SW480 using 83658-2-RR

    SW480 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using RNF216 Recombinant antibody (83658-2-RR)

    WB analysis of Caco-2 using 83658-2-RR

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SW480 cells using RNF216 Recombinant antibody (83658-2-RR)

    IP experiment of SW480 using 83658-2-RR

    IP result of anti-RNF216 (IP:83658-2-RR, 4ug; Detection:83658-2-RR 1:1000) with SW480 cells lysate 1280 ug.

  • Flow cytometry (FC) experiment of A431 cells using RNF216 Recombinant antibody (83658-2-RR)

    FC experiment of A431 using 83658-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RNF216 Recombinant antibody (83658-2-RR, Clone:240489G2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83658-2-RR

    Affinity and Kinetic Characterization of 83658-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83658-2-RR against Human RNF216 were performed. The affinity constant is below 1 pM.

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RNF216 Recombinant antibody

RNF216 Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 83658-2-RR
Clone No.240489G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Synonyms

Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF216

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSW480 cells, Caco-2 cells
Positive IP detected inSW480 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83658-2-RR targets RNF216 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF216 fusion protein Ag0238 相同性解析による交差性が予測される生物種
Full Name ring finger protein 216
Calculated molecular weight 48 kDa
Observed molecular weight~130 kDa
GenBank accession numberBC000787
Gene Symbol RNF216
Gene ID (NCBI) 54476
RRIDAB_3671266
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NWF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively. Western analysis detected the ~130 kDa band as indicated in literature (PMID: 21270397).

Protocols

Product Specific Protocols
WB protocol for RNF216 antibody 83658-2-RRDownload protocol
IP protocol for RNF216 antibody 83658-2-RRDownload protocol
FC protocol for RNF216 antibody 83658-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols