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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using RNF217 Polyclonal antibody (26084-1-AP)

    IHC staining of human liver using 26084-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using RNF217 Polyclonal antibody (26084-1-AP)

    IHC staining of human liver using 26084-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF217 Polyclonal antibody (26084-1-AP)

    IF Staining of HepG2 using 26084-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF217 antibody (26084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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RNF217 Polyclonal antibody

RNF217 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 26084-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

C6orf172, IBRDC1, ring finger protein 217, RNF217

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26084-1-AP targets RNF217 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF217 fusion protein Ag22022 相同性解析による交差性が予測される生物種
Full Name ring finger protein 217
Calculated molecular weight 275 aa, 32 kDa
Observed molecular weight 59, 32 kDa
GenBank accession numberBC026087
Gene Symbol RNF217
Gene ID (NCBI) 154214
RRIDAB_3085839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

E3 ubiquitin-protein ligase RNF217, also known as IBR domain-containing protein 1 (IBRDC1), as a transmembrane (TM) domain-containing RBR-type E3 ubiquitin-protein ligase, belongs to a highly conserved member of the RING finger family. RNF217 mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis(Uniprot, PMID: 33895792).

Protocols

Product Specific Protocols
IHC protocol for RNF217 antibody 26084-1-APDownload protocol
IF protocol for RNF217 antibody 26084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols