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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF25 Polyclonal antibody

RNF25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24536-1-AP

Synonyms

AO7, ring finger protein 25, RNF25

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using RNF25 Polyclonal antibody (24536-1-AP)

    WB analysis of HeLa using 24536-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24536-1-AP (RNF25 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RNF25 Polyclonal antibody (24536-1-AP)

    IHC staining of human cervical cancer using 24536-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24536-1-AP (RNF25 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RNF25 Polyclonal antibody (24536-1-AP)

    IHC staining of human cervical cancer using 24536-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24536-1-AP (RNF25 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24536-1-AP targets RNF25 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF25 fusion protein Ag21369 相同性解析による交差性が予測される生物種
Full Name ring finger protein 25
Calculated molecular weight 459 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC015612
Gene symbol RNF25
Gene ID (NCBI) 64320
RRIDAB_2879594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF25 antibody 24536-1-APDownload protocol
IHC protocol for RNF25 antibody 24536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols