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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230446E9

Cat no : 83137-4-RR

Synonyms

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF31 / HOIP Recombinant antibody (83137-4-RR)

    WB analysis using 83137-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83137-4-RR (RNF31 / HOIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-3 cells using RNF31 / HOIP Recombinant antibody (83137-4-RR)

    FC experiment of PC-3 using 83137-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug RNF31 / HOIP Recombinant antibody (83137-4-RR, Clone:230446E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83137-4-RR is RNF31 / HOIP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF31 / HOIP fusion protein Ag34505 相同性解析による交差性が予測される生物種
Full Name ring finger protein 31
Calculated molecular weight 1072 aa, 120 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC017376
Gene symbol RNF31
Gene ID (NCBI) 55072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF31 / HOIP antibody 83137-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols