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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF40 Recombinant antibody (84058-4-RR)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RNF40 Recombinant antibody (84058-4-RR)

    WB analysis using 84058-4-RR (same clone as 84058-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-4-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF40 Recombinant antibody (84058-4-RR)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF40 Recombinant antibody (84058-4-RR)

    IHC staining of mouse testis using 84058-4-RR (same clone as 84058-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84058-4-RR (RNF40 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84058-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84058-4-RR

    Affinity and Kinetic Characterization of 84058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-4-RR against Human RNF40 were performed. The affinity constant is 1.34 nM.

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RNF40 Recombinant antibody, PBS Only

RNF40 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84058-4-PBS
Clone No.241214D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84058-4-PBS targets RNF40 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF40 fusion protein Ag8047 相同性解析による交差性が予測される生物種
Full Name ring finger protein 40
Calculated molecular weight 114 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC006133
Gene Symbol RNF40
Gene ID (NCBI) 9810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75150
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].