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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF41 Polyclonal antibody

RNF41 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17233-1-AP

Synonyms

FLRF, NRDP1, ring finger protein 41, RNF41, SBBI03

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using RNF41 Polyclonal antibody (17233-1-AP)

    WB analysis of human testis using 17233-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17233-1-AP (RNF41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using RNF41 Polyclonal antibody (17233-1-AP)

    WB analysis of human testis using 17233-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17233-1-AP (RNF41 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RNF41 Polyclonal antibody (17233-1-AP)

    IHC staining of human liver cancer using 17233-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17233-1-AP (RNF41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RNF41 Polyclonal antibody (17233-1-AP)

    IHC staining of human liver cancer using 17233-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17233-1-AP (RNF41 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17233-1-AP is RNF41 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF41 fusion protein Ag11076 相同性解析による交差性が予測される生物種
Full Name ring finger protein 41
Calculated molecular weight 317 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC032637
Gene symbol RNF41
Gene ID (NCBI) 10193
RRIDAB_2878365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF41 antibody 17233-1-APDownload protocol
IHC protocol for RNF41 antibody 17233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB

Front Cardiovasc Med

Deficiency of a novel lncRNA-HRAT protects against myocardial ischemia reperfusion injury by targeting miR-370-3p/RNF41 pathway

Authors - Xinbin Zheng
View Article