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  • Featured Product
  • KD/KO Validated

RNF6 Polyclonal antibody, PBS Only

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20437-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of PC-3 using 20437-1-AP (same clone as 20437-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of PC-3 using 20437-1-AP (same clone as 20437-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using RNF6 Polyclonal antibody (20437-1-AP)

    IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF6 Polyclonal antibody (20437-1-AP)

    IF Staining of HepG2 using 20437-1-AP (same clone as 20437-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using RNF6 antibody 20437-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20437-1-PBS targets RNF6 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF6 fusion protein Ag14209 相同性解析による交差性が予測される生物種
Full Name ring finger protein (C3H2C3 type) 6
Calculated molecular weight 685 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC034688
Gene symbol RNF6
Gene ID (NCBI) 6049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].