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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of PC-3 using 20437-1-AP (same clone as 20437-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of PC-3 using 20437-1-AP (same clone as 20437-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using RNF6 Polyclonal antibody (20437-1-AP)

    IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SW480 cells using RNF6 Polyclonal antibody (20437-1-AP)

    IF Staining of SW480 using 20437-1-AP (same clone as 20437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RNF6 Polyclonal antibody, PBS Only

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20437-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

SPG2, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20437-1-PBS targets RNF6 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF6 fusion protein Ag14209 相同性解析による交差性が予測される生物種
Full Name ring finger protein (C3H2C3 type) 6
Calculated molecular weight 685 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC034688
Gene Symbol RNF6
Gene ID (NCBI) 6049
RRIDAB_10666853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y252
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].