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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNFT2 Polyclonal antibody

RNFT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25685-1-AP

Synonyms

RNFT2, TMEM118

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RNFT2 Polyclonal antibody (25685-1-AP)

    WB analysis of HepG2 using 25685-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25685-1-AP (RNFT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using RNFT2 Polyclonal antibody (25685-1-AP)

    WB analysis of SMMC-7721 using 25685-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25685-1-AP (RNFT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RNFT2 Polyclonal antibody (25685-1-AP)

    WB analysis of HepG2 using 25685-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25685-1-AP (RNFT2 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25685-1-AP is RNFT2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNFT2 fusion protein Ag22313 相同性解析による交差性が予測される生物種
Full Name ring finger protein, transmembrane 2
Calculated molecular weight 444 aa, 49 kDa
Observed molecular weight 49-59 kDa
GenBank accession numberBC011878
Gene symbol RNFT2
Gene ID (NCBI) 84900
RRIDAB_2880194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNFT2 antibody 25685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols