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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using RNGTT Recombinant antibody (83974-4-RR)

    IHC staining of human placenta using 83974-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RNGTT Recombinant antibody (83974-4-RR)

    IHC staining of human placenta using 83974-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNGTT Recombinant antibody (83974-4-RR)

    IF Staining of HepG2 using 83974-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNGTT antibody (83974-4-RR, Clone: 240927H2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using RNGTT Recombinant antibody (83974-4-RR)

    FC experiment of HepG2 using 83974-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-4-RR, Clone:240927H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83974-4-RR

    Affinity and Kinetic Characterization of 83974-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-4-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

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RNGTT Recombinant antibody

RNGTT Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83974-4-RR
Clone No.240927H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83974-4-RR targets RNGTT in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNGTT fusion protein Ag3105 相同性解析による交差性が予測される生物種
Full Name RNA guanylyltransferase and 5'-phosphatase
Calculated molecular weight 597 aa, 69 kDa
GenBank accession numberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
RRIDAB_3671550
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IHC protocol for RNGTT antibody 83974-4-RRDownload protocol
IF protocol for RNGTT antibody 83974-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols