Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10345-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 10345-1-AP
WB result of RNH1 antibody (10345-1-AP, 1:2500) with si-control and si-RNH1 transfected HeLa cells.
WB analysis using 10345-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10345-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10345-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 10345-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 10345-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10345-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10345-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 10345-1-AP
IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:500) with mouse liver tissue lysate 6400ug.
IP experiment of HEK-293T using 10345-1-AP
IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with HEK-293T cells lysate 2040 ug.
IP experiment of mouse liver using 10345-1-AP
IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.
IP experiment of rat liver using 10345-1-AP
IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with rat liver tissue lysate 5520 ug.
IHC staining of mouse liver using 10345-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10345-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10345-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RNH1 antibody (10345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of MCF-7 using 10345-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RNH1 (10345-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

RNH1 Polyclonal antibody

RNH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10345-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

RNH, Ribonuclease/angiogenin inhibitor 1, Ribonuclease inhibitor, RAI, PRI

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, human brain tissue, human heart tissue, human liver tissue, L02 cells, HepG2 cells, Jurkat cells, human placenta tissue
Positive IP detected in	mouse liver tissue, HEK-293T cells, rat liver tissue
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

10345-1-AP targets RNH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RNH1 fusion protein Ag0350 相同性解析による交差性が予測される生物種
Full Name	ribonuclease/angiogenin inhibitor 1
Calculated molecular weight	50 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC000677
Gene Symbol	RNH1
Gene ID (NCBI)	6050
RRID	AB_2269733
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13489
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for RNH1 antibody 10345-1-AP	Download protocol
IHC protocol for RNH1 antibody 10345-1-AP	Download protocol
IF protocol for RNH1 antibody 10345-1-AP	Download protocol
IP protocol for RNH1 antibody 10345-1-AP	Download protocol
FC protocol for RNH1 antibody 10345-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	EMBO J Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis. Authors - Rongpan Bai View Article
human	WB	J Cell Biol Angiogenin cleaves tRNA and promotes stress-induced translational repression. Authors - Yamasaki Satoshi S KD Validated View Article
human	WB	Proc Natl Acad Sci U S A Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers. Authors - Shozo Honda View Article
human	WB	Int J Mol Sci RTCB Complex Regulates Stress-Induced tRNA Cleavage Authors - Yasutoshi Akiyama View Article
human	IHC	J Proteome Res Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE. Authors - Stephens Andrew N AN View Article
mouse	WB	J Biol Chem Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress. Authors - Saikia Mridusmita M View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10345-1-AP

WB analysis of HeLa cells using 10345-1-AP

WB analysis using 10345-1-AP

WB analysis of L02 using 10345-1-AP

WB analysis of mouse liver using 10345-1-AP

WB analysis using 10345-1-AP

WB analysis of human liver using 10345-1-AP

WB analysis of human brain using 10345-1-AP

WB analysis of human heart using 10345-1-AP

IP experiment of mouse liver using 10345-1-AP

IP experiment of HEK-293T using 10345-1-AP

IP experiment of mouse liver using 10345-1-AP

IP experiment of rat liver using 10345-1-AP

IHC staining of mouse liver using 10345-1-AP

IHC staining of mouse liver using 10345-1-AP

IF Staining of MCF-7 using 10345-1-AP

FC experiment of MCF-7 using 10345-1-AP

RNH1 Polyclonal antibody

RNH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Angiogenin cleaves tRNA and promotes stress-induced translational repression.

Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.

RTCB Complex Regulates Stress-Induced tRNA Cleavage

Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.

Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.