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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNH1 Monoclonal antibody (66028-1-Ig)

    WB analysis of HeLa using 66028-1-Ig

    HeLa, Jurkat, L02 cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RNH1 Monoclonal antibody (66028-1-Ig)

    WB analysis of HeLa cells using 66028-1-Ig

    WB result of RNH1 antibody (66028-1-Ig, 1:4000) with si-control and si-RNH1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using RNH1 Monoclonal antibody (66028-1-Ig)

    WB analysis of HeLa using 66028-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RNH1 Monoclonal antibody (66028-1-Ig)

    WB analysis of Jurkat using 66028-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using RNH1 Monoclonal antibody (66028-1-Ig)

    WB analysis of L02 using 66028-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

RNH1 Monoclonal antibody

RNH1 Monoclonal Antibody for WB, ELISA
Cat No. 66028-1-Ig
Clone No.4A1C4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH, RNH1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66028-1-Ig targets RNH1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RNH1 fusion protein Ag18093 相同性解析による交差性が予測される生物種
Full Name ribonuclease/angiogenin inhibitor 1
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000677
Gene Symbol RNH1
Gene ID (NCBI) 6050
RRIDAB_11043338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13489
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for RNH1 antibody 66028-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols