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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ROBO1 Polyclonal antibody

ROBO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25181-1-AP

Synonyms

Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, Roundabout homolog 1, SAX3

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using ROBO1 Polyclonal antibody (25181-1-AP)

    WB analysis of Y79 using 25181-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ROBO1 Polyclonal antibody (25181-1-AP)

    WB analysis of HeLa using 25181-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ROBO1 Polyclonal antibody (25181-1-AP)

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using ROBO1 Polyclonal antibody (25181-1-AP)

    IHC staining of human brain using 25181-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inY79 cells, HeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25181-1-AP is ROBO1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROBO1 fusion protein Ag16461 相同性解析による交差性が予測される生物種
Full Name roundabout, axon guidance receptor, homolog 1 (Drosophila)
Calculated molecular weight 1651 aa, 181 kDa
Observed molecular weight 200-250 kDa
GenBank accession numberBC112336
Gene symbol ROBO1
Gene ID (NCBI) 6091
RRIDAB_2879946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.

Protocols

Product Specific Protocols
WB protocol for ROBO1 antibody 25181-1-APDownload protocol
IHC protocol for ROBO1 antibody 25181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols