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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ROBO2 Polyclonal antibody (21635-1-AP)

    WB analysis using 21635-1-AP (same clone as 21635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21635-1-AP (ROBO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21635-1-PBS in a different storage buffer formulation.

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ROBO2 Polyclonal antibody, PBS Only

ROBO2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 21635-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21635-1-PBS targets ROBO2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROBO2 fusion protein Ag16261 相同性解析による交差性が予測される生物種
Full Name roundabout, axon guidance receptor, homolog 2 (Drosophila)
Calculated molecular weight 1378 aa, 151 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC146772
Gene Symbol ROBO2
Gene ID (NCBI) 6092
RRIDAB_2878893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCK4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.