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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ROBO3 Polyclonal antibody

ROBO3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11982-1-AP

Synonyms

HGPPS, HGPS, RBIG1, RIG1, ROBO3, Roundabout homolog 3, Roundabout like protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ROBO3 Polyclonal antibody (11982-1-AP)

    WB analysis using 11982-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11982-1-AP (ROBO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inNeuro-2a cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11982-1-AP targets ROBO3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROBO3 fusion protein Ag2608 相同性解析による交差性が予測される生物種
Full Name roundabout, axon guidance receptor, homolog 3 (Drosophila)
Calculated molecular weight 148 kDa
Observed molecular weight200-250 kDa, 148 kDa
GenBank accession numberBC008623
Gene symbol ROBO3
Gene ID (NCBI) 64221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS).

Protocols

Product Specific Protocols
WB protocol for ROBO3 antibody 11982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols