Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
66782-1-PBS targets ROCK1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ROCK1 fusion protein Ag16317 相同性解析による交差性が予測される生物種 |
| Full Name | Rho-associated, coiled-coil containing protein kinase 1 |
| Calculated molecular weight | 1354 aa, 158 kDa |
| Observed molecular weight | 150-160 kDa |
| GenBank accession number | BC113114 |
| Gene Symbol | ROCK1 |
| Gene ID (NCBI) | 6093 |
| RRID | AB_2882127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13464 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.