ROCK1 Recombinant antibody
ROCK1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230423C6
Cat no : 83166-5-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, NIH/3T3 cells, HeLa cells, A431 cells, RAW 264.7 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83166-5-RR is ROCK1 Fusion Protein expressed in E. coli.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ROCK1 fusion protein Ag16317 相同性解析による交差性が予測される生物種 |
Full Name | Rho-associated, coiled-coil containing protein kinase 1 |
Calculated molecular weight | 1354 aa, 158 kDa |
Observed molecular weight | 150-160 kDa |
GenBank accession number | BC113114 |
Gene symbol | ROCK1 |
Gene ID (NCBI) | 6093 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
Protocols
Product Specific Protocols | |
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WB protocol for ROCK1 antibody 83166-5-RR | Download protocol |
IF protocol for ROCK1 antibody 83166-5-RR | Download protocol |
FC protocol for ROCK1 antibody 83166-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |