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  • Featured Product
  • KD/KO Validated

ROCK2 Monoclonal antibody, PBS Only

ROCK2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7B8

Cat no : 66633-1-PBS

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ROCK2 Monoclonal antibody (66633-1-Ig)

    WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ROCK2 Monoclonal antibody (66633-1-Ig)

    WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ROCK2 Monoclonal antibody (66633-1-Ig)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ROCK2 Monoclonal antibody (66633-1-Ig)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66633-1-PBS targets ROCK2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ROCK2 fusion protein Ag16330 相同性解析による交差性が予測される生物種
Full Name Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight 77 kDa, 161 kDa
Observed molecular weight 161 kDa
GenBank accession numberBC111801
Gene symbol ROCK2
Gene ID (NCBI) 9475
RRIDAB_2881992
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.