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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    WB analysis of mouse brain using 20248-1-AP (same clone as 20248-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    WB analysis of mouse brain using 20248-1-AP (same clone as 20248-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    WB analysis of rat brain using 20248-1-AP (same clone as 20248-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    IHC staining of human brain using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    IHC staining of human brain using 20248-1-AP (same clone as 20248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ROCK2-Specific(C-term) Polyclonal antibody (20248-1-AP)

    IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ROCK2-Specific(C-term) Polyclonal antibody, PBS Only

ROCK2-Specific(C-term) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20248-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20248-1-PBS targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight 161 kDa
Observed molecular weight 164 kDa
GenBank accession numberNM_004850
Gene Symbol ROCK2
Gene ID (NCBI) 9475
RRIDAB_10665551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.