Validation Data Gallery

Filter: AllWBIHC

WB analysis of HL-60 using 14027-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14027-1-AP
Various lysates subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14027-1-AP
WB result of ROD1 antibody (14027-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROD1 transfected HeLa cells.
WB analysis using 14027-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 14027-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 14027-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14027-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 14027-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lymphoma using 14027-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

ROD1 Polyclonal antibody

ROD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14027-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

PTBP3, Regulator of differentiation 1, ROD1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

14027-1-AP targets ROD1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ROD1 fusion protein Ag5172 相同性解析による交差性が予測される生物種
Full Name	ROD1 regulator of differentiation 1 (S. pombe)
Calculated molecular weight	60 kDa
Observed molecular weight	55-59 kDa
GenBank accession number	BC044585
Gene Symbol	ROD1
Gene ID (NCBI)	9991
RRID	AB_2182321
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95758
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ROD1 is a RNA-binding protein that mediates pre-mRNA alternative splicing regulation, it plays a role in the regulation of cell proliferation, differentiation and migration. ROD1 exist some isoforms and also can be modified with the molecular weight range from 55-59 kDa.

Protocols

Product Specific Protocols
WB protocol for ROD1 antibody 14027-1-AP	Download protocol
IHC protocol for ROD1 antibody 14027-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Res The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes. Authors - Juan Chen KO Validated View Article
		iScience Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis Authors - Qianqing Wang View Article
human	WB	Cancer Cell Int PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression. Authors - Yingji Chen View Article
human	WB,IHC	Cancer Cell Int PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway. Authors - Qiong Wu View Article
human	WB	Oncol Lett Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus. Authors - Ya Zhou View Article
mouse	WB	Mol Cell PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells Authors - Camilla Iannone View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HL-60 using 14027-1-AP

WB analysis using 14027-1-AP

WB analysis of HeLa using 14027-1-AP

WB analysis using 14027-1-AP

WB analysis using 14027-1-AP

WB analysis using 14027-1-AP

WB analysis of mouse thymus using 14027-1-AP

IHC staining of human lymphoma using 14027-1-AP

IHC staining of human lymphoma using 14027-1-AP

ROD1 Polyclonal antibody

ROD1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.

Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis

PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.

PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway.

Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus.

PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells