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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ROR1 Monoclonal antibody (66923-1-Ig)

    WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ROR1 Monoclonal antibody (66923-1-Ig)

    WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ROR1 Monoclonal antibody (66923-1-Ig)

    WB analysis of NIH/3T3 using 66923-1-Ig (same clone as 66923-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ROR1 Monoclonal antibody (66923-1-Ig)

    WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ROR1 Monoclonal antibody (66923-1-Ig)

    IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ROR1 Monoclonal antibody (66923-1-Ig)

    IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51139-1

    Cytometric bead array standard curve of MP51139-1

    Cytometric bead array standard curve of MP51139-1, ROR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66923-2-PBS. Detection antibody: 66923-1-PBS. Standard:Ag14586. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP51139-2

    Cytometric bead array standard curve of MP51139-2

    Cytometric bead array standard curve of MP51139-2, ROR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66923-3-PBS. Detection antibody: 66923-1-PBS. Standard:Ag14586. Range: 0.781-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

ROR1 Monoclonal antibody, PBS Only (Detector)

ROR1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66923-1-PBS
Clone No.1F5D8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

NTRKR1, Neurotrophic tyrosine kinase, receptor-related 1, Inactive tyrosine-protein kinase transmembrane receptor ROR1, 1F5D8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66923-1-PBS targets ROR1 as part of a matched antibody pair:

MP51139-1: 66923-2-PBS capture and 66923-1-PBS detection (validated in Cytometric bead array)

MP51139-2: 66923-3-PBS capture and 66923-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ROR1 fusion protein Ag14586 相同性解析による交差性が予測される生物種
Full Name receptor tyrosine kinase-like orphan receptor 1
Calculated molecular weight 937 aa, 104 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC006374
Gene Symbol ROR1
Gene ID (NCBI) 4919
RRIDAB_2882250
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01973
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ROR1 is a member of the RTK family of orphan receptors related to muscle-specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937 amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain, and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).