Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 17635-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 17635-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 17635-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17635-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17635-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17635-1-AP
IP result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:300) with mouse brain tissue lysate 3440ug.
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 40x lens).

RORB Polyclonal antibody

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17635-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Synonyms

NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Y79 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 3 publications below

Product Information

17635-1-AP targets RORB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RORB fusion protein Ag11910 相同性解析による交差性が予測される生物種
Full Name	RAR-related orphan receptor B
Calculated molecular weight	459 aa, 52 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC051830
Gene Symbol	RORB
Gene ID (NCBI)	6096
RRID	AB_2180290
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92753
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

Protocols

Product Specific Protocols
WB protocol for RORB antibody 17635-1-AP	Download protocol
IHC protocol for RORB antibody 17635-1-AP	Download protocol
IP protocol for RORB antibody 17635-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Neuron Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia. Authors - Chao Wang View Article
mouse	IF	PLoS Biol An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold Authors - Wenny Wong View Article
mouse	IF	eNeuro Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment. Authors - Natalia Galindo-Riera View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Y79 using 17635-1-AP

WB analysis using 17635-1-AP

WB analysis of Y79 using 17635-1-AP

WB analysis of SH-SY5Y using 17635-1-AP

WB analysis of SH-SY5Y using 17635-1-AP

WB analysis of Y79 using 17635-1-AP

WB analysis of Y79 using 17635-1-AP

WB analysis of mouse brain using 17635-1-AP

WB analysis of mouse brain using 17635-1-AP

IP experiment of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

RORB Polyclonal antibody

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.

An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold

Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.