RORB Polyclonal antibody

Name: RORB Polyclonal antibody
Brand: Proteintech
SKU: 17635-1-AP
Rating: 1.00 (1 reviews)

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 17635-1-AP

Synonyms

NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 17635-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 17635-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 17635-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 17635-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17635-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17635-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17635-1-AP
IP result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:300) with mouse brain tissue lysate 3440ug.
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of mouse brain using 17635-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 17635-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Y79 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 3 publications below

Product Information

17635-1-AP targets RORB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RORB fusion protein Ag11910 相同性解析による交差性が予測される生物種
Full Name	RAR-related orphan receptor B
Calculated molecular weight	459 aa, 52 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC051830
Gene symbol	RORB
Gene ID (NCBI)	6096
RRID	AB_2180290
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

Protocols

Product Specific Protocols
WB protocol for RORB antibody 17635-1-AP	Download protocol
IHC protocol for RORB antibody 17635-1-AP	Download protocol
IP protocol for RORB antibody 17635-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Neuron Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia. Authors - Chao Wang View Article
mouse	IF	PLoS Biol An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold Authors - Wenny Wong View Article
mouse	IF	eNeuro Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment. Authors - Natalia Galindo-Riera View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Etienne (Verified Customer) (12-19-2022)

Did not work at all in human brain organoids (where the target is present)

Applications: Immunofluorescence
Primary Antibody Dilution: 1/50, 1/200, 1/500
Cell Tissue Type: Human Brain Organoids

RORB Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:17635-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RORB Polyclonal antibody

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of Y79 using 17635-1-AP

WB analysis using 17635-1-AP

WB analysis of Y79 using 17635-1-AP

WB analysis of Y79 using 17635-1-AP

WB analysis of SH-SY5Y using 17635-1-AP

WB analysis of SH-SY5Y using 17635-1-AP

WB analysis of Y79 using 17635-1-AP

WB analysis of mouse brain using 17635-1-AP

WB analysis of mouse brain using 17635-1-AP

IP experiment of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

IHC staining of mouse brain using 17635-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.

An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold

Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.

Reviews