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- KD/KO Validated
Kazrin Polyclonal antibody
Kazrin Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11572-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skin tissue, mouse heart tissue, human heart tissue, mouse brain tissue, rat heart tissue, rat skin tissue |
Positive IHC detected in | human colon cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
11572-1-AP targets Kazrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Kazrin fusion protein Ag2152 相同性解析による交差性が予測される生物種 |
Full Name | kazrin |
Calculated molecular weight | 415 aa, 46 kDa |
Observed molecular weight | 46 kDa, 37 kDa |
GenBank accession number | BC035501 |
Gene symbol | Kazrin |
Gene ID (NCBI) | 23254 |
RRID | AB_833126 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin exists three isoforms:kazrin A (46.7 kDa), kazrin B (46 kDa), and kazrin C (37 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for Kazrin antibody 11572-1-AP | Download protocol |
IHC protocol for Kazrin antibody 11572-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Invest Dermatol Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.
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