Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 10847-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 10847-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10847-1-AP (MST4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10847-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10847-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10847-1-AP
IP result of anti-MST4 (IP:10847-1-AP, 4ug; Detection:10847-1-AP 1:500) with MCF-7 cells lysate 2400ug.
IHC staining of human liver using 10847-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10847-1-AP (MST4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 10847-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10847-1-AP (MST4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10847-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of MCF-7 using 10847-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MST4 antibody (10847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

MST4 Polyclonal antibody

MST4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10847-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

STK26, MST3 and SOK1-related kinase, Mst3 and SOK1 related kinase, MST 4, MASK

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, Jurkat cells, HL-60 cells, MCF-7 cells, L02 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human liver tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 3 publications below
IF	See 4 publications below

Product Information

10847-1-AP targets MST4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MST4 fusion protein Ag1302 相同性解析による交差性が予測される生物種
Full Name	serine/threonine protein kinase MST4
Calculated molecular weight	16 kDa, 46 kDa
Observed molecular weight	47-50 kDa
GenBank accession number	BC017213
Gene Symbol	MST4
Gene ID (NCBI)	51765
RRID	AB_513907
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P289
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MST4 antibody 10847-1-AP	Download protocol
IHC protocol for MST4 antibody 10847-1-AP	Download protocol
IF protocol for MST4 antibody 10847-1-AP	Download protocol
IP protocol for MST4 antibody 10847-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	J Cell Sci CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division. Authors - Eric Peterman KD Validated View Article
human	WB,IHC,IF	Int J Oncol MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway. Authors - Zhen-Hai Lin KD Validated View Article
mouse	WB,IF	Brain Res Bull MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice. Authors - Xiaodong Wu View Article
human	IHC	Onco Targets Ther MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression. Authors - Hanxi Yu View Article
mouse	WB,IF	Behav Neurol MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice. Authors - Xiaodong Wu View Article
human	WB	Antiviral Res Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3 Authors - Tan Ming View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 10847-1-AP

WB analysis using 10847-1-AP

WB analysis of Jurkat using 10847-1-AP

WB analysis of HL-60 using 10847-1-AP

IP experiment of MCF-7 using 10847-1-AP

IHC staining of human liver using 10847-1-AP

IHC staining of human liver using 10847-1-AP

IHC staining of human colon cancer using 10847-1-AP

IF Staining of MCF-7 using 10847-1-AP

MST4 Polyclonal antibody

MST4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.

MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.

MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice.

MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression.

MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.

Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3