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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RP9 Polyclonal antibody

RP9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16832-1-AP

Synonyms

PAP 1, Pim 1 associated protein, Retinitis pigmentosa 9 protein, RP9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RP9 Polyclonal antibody (16832-1-AP)

    WB analysis of HeLa using 16832-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RP9 Polyclonal antibody (16832-1-AP)

    WB analysis of mouse brain using 16832-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RP9 Polyclonal antibody (16832-1-AP)

    WB analysis of MCF-7 using 16832-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RP9 Polyclonal antibody (16832-1-AP)

    WB analysis of HeLa using 16832-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RP9 Polyclonal antibody (16832-1-AP)

    WB analysis of HepG2 using 16832-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RP9 Polyclonal antibody (16832-1-AP)

    WB analysis of MCF-7 using 16832-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16832-1-AP targets RP9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RP9 fusion protein Ag10441 相同性解析による交差性が予測される生物種
Full Name retinitis pigmentosa 9 (autosomal dominant)
Calculated molecular weight 221 aa, 26 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC025928
Gene symbol RP9
Gene ID (NCBI) 6100
RRIDAB_2878322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RP9 antibody 16832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.

Authors - Karl Bertram
View Article