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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of pig spleen using 67999-1-Ig (same clone as 67999-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of rat testis using 67999-1-Ig (same clone as 67999-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of mouse testis using 67999-1-Ig (same clone as 67999-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit spleen tissue using RPA2 Monoclonal antibody (67999-1-Ig)

    WB analysis of rabbit spleen using 67999-1-Ig (same clone as 67999-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RPA2 Monoclonal antibody (67999-1-Ig)

    IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

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RPA2 Monoclonal antibody, PBS Only

RPA2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67999-1-PBS
Clone No.1B9H11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67999-1-PBS targets RPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPA2 fusion protein Ag24783 相同性解析による交差性が予測される生物種
Full Name replication protein A2, 32kDa
Calculated molecular weight 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_2918747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.