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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPAIN Polyclonal antibody

RPAIN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15220-1-AP

Synonyms

HRIP, RIP, RPA interacting protein, RPAIN

Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using RPAIN Polyclonal antibody (15220-1-AP)

    WB analysis of A2780 using 15220-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using RPAIN Polyclonal antibody (15220-1-AP)

    WB analysis of A375 using 15220-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RPAIN Polyclonal antibody (15220-1-AP)

    WB analysis of mouse ovary using 15220-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A375 cells using RPAIN Polyclonal antibody (15220-1-AP)

    IP experiment of A375 using 15220-1-AP

    IP result of anti-RPAIN (IP:15220-1-AP, 4ug; Detection:15220-1-AP 1:300) with A375 cells lysate 2200 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RPAIN Polyclonal antibody (15220-1-AP)

    IHC staining of human ovary tumor using 15220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RPAIN Polyclonal antibody (15220-1-AP)

    IHC staining of human ovary tumor using 15220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inA375 cells, mouse ovary tissue, A2780 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15220-1-AP targets RPAIN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPAIN fusion protein Ag7392 相同性解析による交差性が予測される生物種
Full Name RPA interacting protein
Calculated molecular weight 25 kDa
Observed molecular weight 30-45 kDa
GenBank accession numberBC004451
Gene symbol RPAIN
Gene ID (NCBI) 84268
RRIDAB_2180976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for RPAIN antibody 15220-1-APDownload protocol
IHC protocol for RPAIN antibody 15220-1-APDownload protocol
IP protocol for RPAIN antibody 15220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols