RPAP3 Polyclonal antibody

Name: RPAP3 Polyclonal antibody
Brand: Proteintech
SKU: 23741-1-AP

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23741-1-AP

Synonyms

RNA polymerase II-associated protein 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 23741-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 23741-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23741-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 23741-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 23741-1-AP
IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug.
IHC staining of human stomach cancer using 23741-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 23741-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 23741-1-AP
Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 23741-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

23741-1-AP targets RPAP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPAP3 fusion protein Ag20661 相同性解析による交差性が予測される生物種
Full Name	RNA polymerase II associated protein 3
Calculated molecular weight	76 kDa
Observed molecular weight	76 kDa
GenBank accession number	BC056415
Gene symbol	RPAP3
Gene ID (NCBI)	79657
RRID	AB_2879313
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RPAP3 antibody 23741-1-AP	Download protocol
IHC protocol for RPAP3 antibody 23741-1-AP	Download protocol
IF protocol for RPAP3 antibody 23741-1-AP	Download protocol
IP protocol for RPAP3 antibody 23741-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Cell Rep DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling Authors - Yu-Qian Mao View Article
human	WB	Cell Chem Biol RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Authors - Paul Yenerall View Article
human	WB	Biochem Biophys Res Commun HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma. Authors - Akihiko Nakamura View Article
human	WB	medRxiv New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops Authors - Md Lutfur Rahman View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPAP3 Polyclonal antibody

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 23741-1-AP

WB analysis of Jurkat using 23741-1-AP

WB analysis of HeLa using 23741-1-AP

WB analysis of Jurkat using 23741-1-AP

IP experiment of HEK-293 using 23741-1-AP

IHC staining of human stomach cancer using 23741-1-AP

IHC staining of human stomach cancer using 23741-1-AP

IF Staining of HeLa using 23741-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling

RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.

HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.

New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops