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  • Featured Product
  • KD/KO Validated

RPGRIP1 Polyclonal antibody

RPGRIP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13214-1-AP

Synonyms

CORD9, LCA6, RGI1, RGRIP, RPGR interacting protein 1, RPGRIP, RPGRIP1, RPGRIP1d

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPGRIP1 Polyclonal antibody (13214-1-AP)

    WB analysis using 13214-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPGRIP1 Polyclonal antibody (13214-1-AP)

    WB analysis using 13214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RPGRIP1 Polyclonal antibody (13214-1-AP)

    IHC staining of mouse eye using 13214-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RPGRIP1 Polyclonal antibody (13214-1-AP)

    IHC staining of mouse eye using 13214-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPGRIP1 Polyclonal antibody (13214-1-AP)

    IHC staining of mouse testis using 13214-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RPGRIP1 Polyclonal antibody (13214-1-AP)

    IHC staining of mouse testis using 13214-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, HL-60 cells
Positive IHC detected inmouse eye tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13214-1-AP targets RPGRIP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPGRIP1 fusion protein Ag4089 相同性解析による交差性が予測される生物種
Full Name retinitis pigmentosa GTPase regulator interacting protein 1
Calculated molecular weight 147 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039089
Gene symbol RPGRIP1
Gene ID (NCBI) 57096
RRIDAB_10639367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. The biological functions of RPGRIP1 are complex. Mutations in this gene lead to autosomal recessive congenital blindness. In the eye, it is expressed in amacrine neurons and photoreceptors, and in numerous other tissues, albeit at greatly reduced levels. Moreover, it exists multiple isoforms with species-specific subcellular localization patterns, and the different isoforms perform cell-specific functions. Within photoreceptors, RPGRIP1 localizes predominantly to outer segments (OS) of rods and cones in humans, whereas in bovine eyes, it is found only in rod OS. In contrast, in mice, RPGRIP1 is found primarily in connecting cilia (CC), where it localizes between the ciliary axoneme and the plasma membrane. RPGRIP1 has multiple isoforms with MW 147 kDa, 70-76 kDa, 103 kDa, and 66 kDa.

Protocols

Product Specific Protocols
WB protocol for RPGRIP1 antibody 13214-1-APDownload protocol
IHC protocol for RPGRIP1 antibody 13214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

EMBO J

Cell type-specific regulation of ciliary transition zone assembly in vertebrates.

Authors - Antonia Wiegering
  • KO Validated
View Article