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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPIA Polyclonal antibody

RPIA Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13010-1-AP

Synonyms

RPI, Ribose-5-phosphate isomerase, ribose 5 phosphate isomerase A, Phosphoriboisomerase, EC:5.3.1.6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPIA Polyclonal antibody (13010-1-AP)

    WB analysis using 13010-1-AP

    mouse spleen tissues were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPIA Polyclonal antibody (13010-1-AP)

    WB analysis using 13010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPIA Polyclonal antibody (13010-1-AP)

    IF Staining of HepG2 using 13010-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPIA antibody 13010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse spleen tissue, HEK-293 cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13010-1-AP targets RPIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPIA fusion protein Ag3699 相同性解析による交差性が予測される生物種
Full Name ribose 5-phosphate isomerase A
Calculated molecular weight 311 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC015529
Gene symbol RPIA
Gene ID (NCBI) 22934
RRIDAB_10638301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPIA(Ribose-5-phosphate isomerase) is also named as RPI and belongs to the ribose 5-phosphate isomerase family. It is involved in pentose-phosphate shunt, non-oxidative branch. It is an enzyme in the pentose-phosphate pathway, appears to be determined by a single structural locus and is probably a dimer(PMID:7396409).

Protocols

Product Specific Protocols
WB protocol for RPIA antibody 13010-1-APDownload protocol
IF protocol for RPIA antibody 13010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Int J Biol Sci

Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitination

Authors - Longzheng Xia
View Article