RPL10 Polyclonal antibody

Name: RPL10 Polyclonal antibody
Brand: Proteintech
SKU: 17013-1-AP

RPL10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17013-1-AP

Synonyms

60S ribosomal protein L10, DXS648, DXS648E, Laminin receptor homolog, NOV, Protein QM, QM, ribosomal protein L10, RPL10, Tumor suppressor QM

Validation Data Gallery

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AllWBIHC

WB analysis using 17013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 17013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 17013-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17013-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 17013-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 17013-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 17013-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, COLO 320 cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

17013-1-AP targets RPL10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL10 fusion protein Ag9969 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L10
Calculated molecular weight	25 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC003358
Gene symbol	RPL10
Gene ID (NCBI)	6134
RRID	AB_2181278
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.

Protocols

Product Specific Protocols
WB protocol for RPL10 antibody 17013-1-AP	Download protocol
IHC protocol for RPL10 antibody 17013-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Discov Ribosomal RNA regulates chromosome clustering during mitosis. Authors - Kai Ma View Article
human	WB,IHC	Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. Authors - Xinlu Liu View Article
mouse	IF	Development Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice. Authors - Stephanie Herrlinger View Article
human	WB	Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation Authors - Yu Fan View Article
human	WB	Cardiovasc Res The translational landscape of human vascular smooth muscle cells identifies novel short ORF-encoded peptide regulators for phenotype alteration Authors - Kang Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL10 Polyclonal antibody

RPL10 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 17013-1-AP

WB analysis using 17013-1-AP

IHC staining of mouse brain using 17013-1-AP

IHC staining of mouse brain using 17013-1-AP

IHC staining of human liver using 17013-1-AP

IHC staining of human liver using 17013-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ribosomal RNA regulates chromosome clustering during mitosis.

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice.

GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation

The translational landscape of human vascular smooth muscle cells identifies novel short ORF-encoded peptide regulators for phenotype alteration