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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL10L Polyclonal antibody

RPL10L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16222-1-AP

Synonyms

60S ribosomal protein L10 like, ribosomal protein L10 like, RPL10_5_1358, RPL10L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL10L Polyclonal antibody (16222-1-AP)

    WB analysis using 16222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPL10L Polyclonal antibody (16222-1-AP)

    WB analysis using 16222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, SKOV-3 cells, mouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16222-1-AP targets RPL10L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL10L fusion protein Ag9275 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L10-like
Calculated molecular weight 214 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC014310
Gene symbol RPL10L
Gene ID (NCBI) 140801
RRIDAB_3085482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPL10L (Ribosomal Protein L10 Like), is a testis-specific component of the ribosome, is responsible for regulating the biosynthesis and folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm(Uniprot). RPL10L plays an essential role during meiosis of spermatogenesis by compensating for its X-linked parental paralog, RPL10, during and after meiotic sex chromosome inactivation(MSCI) (PMID: 28502657).

Protocols

Product Specific Protocols
WB protocol for RPL10L antibody 16222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols