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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL10L Polyclonal antibody (16222-1-AP)

    WB analysis using 16222-1-AP (same clone as 16222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPL10L Polyclonal antibody (16222-1-AP)

    WB analysis using 16222-1-AP (same clone as 16222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16222-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16222-1-PBS targets RPL10L in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL10L fusion protein Ag9275 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L10-like
Calculated molecular weight 214 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC014310
Gene Symbol RPL10L
Gene ID (NCBI) 140801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L21
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RPL10L (Ribosomal Protein L10 Like), is a testis-specific component of the ribosome, is responsible for regulating the biosynthesis and folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm(Uniprot). RPL10L plays an essential role during meiosis of spermatogenesis by compensating for its X-linked parental paralog, RPL10, during and after meiotic sex chromosome inactivation(MSCI) (PMID: 28502657).