Validation Data Gallery
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Tested Applications
| Positive WB detected in | human liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 10 publications below |
| WB | See 30 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
16277-1-AP targets RPL11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPL11 fusion protein Ag9356 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L11 |
| Calculated molecular weight | 177 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC018970 |
| Gene Symbol | RPL11 |
| Gene ID (NCBI) | 6135 |
| RRID | AB_2181292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62913 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL11 antibody 16277-1-AP | Download protocol |
| IHC protocol for RPL11 antibody 16277-1-AP | Download protocol |
| IF protocol for RPL11 antibody 16277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling. | ||
Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. | ||
Am J Hum Genet Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer. | ||
Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. | ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. |