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RPL11 Polyclonal antibody

RPL11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16277-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

60S ribosomal protein L11, CLL associated antigen KW 12, DBA7, GIG34, ribosomal protein L11, RPL11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16277-1-AP targets RPL11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL11 fusion protein Ag9356 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L11
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC018970
Gene Symbol RPL11
Gene ID (NCBI) 6135
RRIDAB_2181292
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.

Protocols

Product Specific Protocols
WB protocol for RPL11 antibody 16277-1-APDownload protocol
IHC protocol for RPL11 antibody 16277-1-APDownload protocol
IF protocol for RPL11 antibody 16277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao
mouseIF

Nat Commun

Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Authors - Kai Hao
humanWB

Am J Hum Genet

Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.

Authors - Koji Nakamura
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
humanWB

Blood

Defective ribosome assembly in Shwachman-Diamond syndrome.

Authors - Wong Chi C CC
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
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