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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL12 Polyclonal antibody (14536-1-AP)

    WB analysis using 14536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RPL12 Polyclonal antibody (14536-1-AP)

    WB analysis of Jurkat using 14536-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RPL12 Polyclonal antibody (14536-1-AP)

    WB analysis of A549 using 14536-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of human placenta using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of human placenta using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of human endometrial cancer using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of human endometrial cancer using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of mouse brain using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of mouse stomach using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of rat brain using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RPL12 Polyclonal antibody (14536-1-AP)

    IHC staining of rat stomach using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RPL12 Polyclonal antibody

RPL12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14536-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman placenta tissue, human endometrial cancer tissue, mouse brain tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14536-1-AP targets RPL12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL12 fusion protein Ag6037 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L12
Calculated molecular weight 18 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC050644
Gene Symbol RPL12
Gene ID (NCBI) 6136
RRIDAB_2269722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30050
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL12 antibody 14536-1-APDownload protocol
IHC protocol for RPL12 antibody 14536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

CDK1 couples proliferation with protein synthesis.

Authors - Katharina Haneke
View Article
humanWB

Sci Rep

RNA G-quadruplex structures control ribosomal protein production

Authors - DhavalVarshney
View Article