RPL14 Polyclonal antibody

Name: RPL14 Polyclonal antibody
Brand: Proteintech
SKU: 14991-1-AP

RPL14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14991-1-AP

Synonyms

Large ribosomal subunit protein eL14, L14, hRL14, CTG B33, CAG-ISL 7

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14991-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 14991-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14991-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14991-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14991-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 14991-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14991-1-AP
IP result of anti-RPL14 (IP:14991-1-AP, 4ug; Detection:14991-1-AP 1:1000) with mouse liver tissue lysate 6800ug.
IHC staining of human colon cancer using 14991-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 14991-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon cancer using 14991-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14991-1-AP (RPL14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14991-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14991-1-AP (RPL14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14991-1-AP
Immunofluorescent analysis of HepG2 cells, using RPL14 antibody 14991-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14991-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

14991-1-AP targets RPL14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL14 fusion protein Ag6976 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L14
Calculated molecular weight	23 kDa
Observed molecular weight	25-30 kDa
GenBank accession number	BC000606
Gene symbol	RPL14
Gene ID (NCBI)	9045
RRID	AB_2285319
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

Protocols

Product Specific Protocols
WB protocol for RPL14 antibody 14991-1-AP	Download protocol
IHC protocol for RPL14 antibody 14991-1-AP	Download protocol
IF protocol for RPL14 antibody 14991-1-AP	Download protocol
IP protocol for RPL14 antibody 14991-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. Authors - Xiaochen Gai View Article
human	WB,IF	Cell Discov Ribosomal RNA regulates chromosome clustering during mitosis. Authors - Kai Ma View Article
human	WB	Am J Cancer Res UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc Authors - Dalian Gong View Article
human	CoIP,WB	Cell Discov The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination Authors - Duo Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL14 Polyclonal antibody

RPL14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14991-1-AP

WB analysis using 14991-1-AP

WB analysis of HeLa using 14991-1-AP

WB analysis of MCF-7 using 14991-1-AP

WB analysis of mouse liver using 14991-1-AP

WB analysis of rat liver using 14991-1-AP

IP experiment of mouse liver using 14991-1-AP

IHC staining of human colon cancer using 14991-1-AP

IHC staining of human colon cancer using 14991-1-AP

IHC staining of human colon cancer using 14991-1-AP

IHC staining of human colon cancer using 14991-1-AP

IF Staining of HepG2 using 14991-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Ribosomal RNA regulates chromosome clustering during mitosis.

UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc

The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination