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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL18A Polyclonal antibody, PBS Only

RPL18A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14653-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of PC-3 using 14653-1-AP (same clone as 14653-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of HeLa using 14653-1-AP (same clone as 14653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of human brain using 14653-1-AP (same clone as 14653-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RPL18A Polyclonal antibody (14653-1-AP)

    WB analysis of mouse lung using 14653-1-AP (same clone as 14653-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL18A Polyclonal antibody (14653-1-AP)

    IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL18A Polyclonal antibody (14653-1-AP)

    IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL18A Polyclonal antibody (14653-1-AP)

    IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14653-1-PBS targets RPL18A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL18A fusion protein Ag6251 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L18a
Calculated molecular weight 21 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC066319
Gene symbol RPL18A
Gene ID (NCBI) 6142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.