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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL18A Recombinant antibody (83928-1-RR)

    WB analysis using 83928-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83928-1-RR (RPL18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPL18A Recombinant antibody (83928-1-RR)

    IHC staining of human stomach cancer using 83928-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83928-1-RR (RPL18A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83928-1-RR

    Affinity and Kinetic Characterization of 83928-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83928-1-RR against Human RPL18A were performed. The affinity constant is below 1 pM.

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RPL18A Recombinant antibody

RPL18A Recombinant Antibody for WB, IHC, ELISA
Cat No. 83928-1-RR
Clone No.240841B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, 240841B1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83928-1-RR targets RPL18A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL18A fusion protein Ag6251 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L18a
Calculated molecular weight 21 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC066319
Gene Symbol RPL18A
Gene ID (NCBI) 6142
RRIDAB_3671508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ02543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.

Protocols

Product Specific Protocols
WB protocol for RPL18A antibody 83928-1-RRDownload protocol
IHC protocol for RPL18A antibody 83928-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols