Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16386-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16386-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16386-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16386-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 16386-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16386-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16386-1-AP
IP result of anti-RPL23A (IP:16386-1-AP, 4ug; Detection:16386-1-AP 1:500) with HepG2 cells lysate 3200ug.
IHC staining of human normal colon using 16386-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16386-1-AP (RPL23A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 16386-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16386-1-AP (RPL23A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16386-1-AP
Immunofluorescent analysis of HepG2 cells using 16386-1-AP (RPL23A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RPL23A Polyclonal antibody

RPL23A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16386-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

ribosomal protein L23a, MDA20, Large ribosomal subunit protein uL23, 60S ribosomal protein L23a

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, human liver tissue, human heart tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
RIP	See 1 publications below

Product Information

16386-1-AP targets RPL23A in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL23A fusion protein Ag9424 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L23a
Calculated molecular weight	156 aa, 18 kDa
Observed molecular weight	18-23 kDa
GenBank accession number	BC014459
Gene Symbol	RPL23A
Gene ID (NCBI)	6147
RRID	AB_2269755
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62750
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL23A antibody 16386-1-AP	Download protocol
IHC protocol for RPL23A antibody 16386-1-AP	Download protocol
IF protocol for RPL23A antibody 16386-1-AP	Download protocol
IP protocol for RPL23A antibody 16386-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. Authors - Kaosheng Lv View Article
human	WB	Nat Commun Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs Authors - James C Murphy View Article
mouse	WB,RIP	Nat Commun The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis. Authors - Mehdi Pirouz View Article
human	WB	Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. Authors - Janja Božič View Article
mouse	WB	Gene Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications. Authors - Trieu-Duc Vu View Article
human	WB	Cell Death Dis HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer. Authors - Zhiyuan Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16386-1-AP

WB analysis of human liver using 16386-1-AP

WB analysis of HepG2 using 16386-1-AP

WB analysis of human liver using 16386-1-AP

WB analysis of human heart using 16386-1-AP

WB analysis of HeLa using 16386-1-AP

IP experiment of HepG2 using 16386-1-AP

IHC staining of human normal colon using 16386-1-AP

IHC staining of human normal colon using 16386-1-AP

IF Staining of HepG2 using 16386-1-AP

RPL23A Polyclonal antibody

RPL23A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs

The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis.

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.

HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer.