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KD/KO Validated

RPL26 Polyclonal antibody

Name: RPL26 Polyclonal antibody
Brand: Proteintech
SKU: 17619-1-AP
Rating: 5.00 (1 reviews)

RPL26 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 17619-1-AP

Synonyms

ribosomal protein L26, Large ribosomal subunit protein uL24, 60S ribosomal protein L26

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis of K-562 using 17619-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17619-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17619-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17619-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 17619-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPL26 antibody (17619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 17619-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

17619-1-AP targets RPL26 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL26 fusion protein Ag11821 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L26
Calculated molecular weight	145 aa, 17 kDa
Observed molecular weight	17-22 kDa
GenBank accession number	BC071664
Gene symbol	RPL26
Gene ID (NCBI)	6154
RRID	AB_2878420
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL26 antibody 17619-1-AP	Download protocol
IF protocol for RPL26 antibody 17619-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun PMID: 31792210 Authors - Sunwang Xu View Article
human	WB	Genes (Basel) Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus. Authors - Meijuan Zou KD Validated View Article
human	WB	Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function Authors - Wonyul Jang View Article
human	WB	Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation Authors - Yu Fan View Article
human	WB,IHC,CoIP	Am J Cancer Res UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc Authors - Dalian Gong View Article
human	WB	J Therm Biol Pre-heating stress associated with acute oral leucine supplementation effects in rat gastrocnemius muscle: Implications for protein synthesis signaling pathways Authors - Toshinori Yoshihara View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

H (Verified Customer) (07-10-2020)

This antibody works well. I did crispr knock-in of RPL26 and it was easily detected by this antibody

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: HCT116

RPL26 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:17619-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL26 Polyclonal antibody

RPL26 Polyclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis of K-562 using 17619-1-AP

WB analysis of Jurkat using 17619-1-AP

WB analysis of HeLa using 17619-1-AP

WB analysis of HepG2 using 17619-1-AP

IF Staining of HeLa using 17619-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 31792210

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation

UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc

Pre-heating stress associated with acute oral leucine supplementation effects in rat gastrocnemius muscle: Implications for protein synthesis signaling pathways

Reviews