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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL26L1 Polyclonal antibody

RPL26L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16487-1-AP

Synonyms

RPL26P1, Ribosomal protein uL24-like, ribosomal protein L26 like 1, Large ribosomal subunit protein uL24-like 1, 60S ribosomal protein L26-like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RPL26L1 Polyclonal antibody (16487-1-AP)

    WB analysis of HEK-293 using 16487-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RPL26L1 Polyclonal antibody (16487-1-AP)

    WB analysis of K-562 using 16487-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RPL26L1 Polyclonal antibody (16487-1-AP)

    WB analysis of MCF-7 using 16487-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL26L1 Polyclonal antibody (16487-1-AP)

    WB analysis of HeLa using 16487-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16487-1-AP (RPL26L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RPL26L1 Polyclonal antibody (16487-1-AP)

    IHC staining of human stomach using 16487-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16487-1-AP (RPL26L1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPL26L1 Polyclonal antibody (16487-1-AP)

    IF Staining of HeLa using 16487-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16487-1-AP (RPL26L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16487-1-AP targets RPL26L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL26L1 fusion protein Ag9609 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L26-like 1
Calculated molecular weight 145 aa, 17 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC017360
Gene symbol RPL26L1
Gene ID (NCBI) 51121
RRIDAB_2181740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPL26L1, also named as RPL26P1, belongs to the universal ribosomal protein uL24 family. RPL26L1 shares high sequence similarity with ribosomal protein L26 and plays a role in protein synthesis (PMID: 31673979). The molecular mass of RPL26L1 is 17 kDa. Studies have shown that RPL26L1 is related to autism spectrum disorder and is one of the potential biomarkers of the neurodegenerative disease Friedreich's ataxia. In addition, RPL26L1 may be closely related with SLE development (PMID: 31889427).

Protocols

Product Specific Protocols
WB protocol for RPL26L1 antibody 16487-1-APDownload protocol
IHC protocol for RPL26L1 antibody 16487-1-APDownload protocol
IF protocol for RPL26L1 antibody 16487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Brain Sci

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest

Authors - Qiang Zhang
View Article