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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RPL27A Polyclonal antibody (16002-1-AP)

    WB analysis of A549 using 16002-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of A549 cells using RPL27A Polyclonal antibody (16002-1-AP)

    WB analysis of A549 using 16002-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL27A Polyclonal antibody (16002-1-AP)

    IHC staining of mouse stomach using 16002-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL27A Polyclonal antibody (16002-1-AP)

    IHC staining of mouse stomach using 16002-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RPL27A Polyclonal antibody (16002-1-AP)

    IF Staining of A549 using 16002-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 16002-1-AP (RPL27A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

RPL27A Polyclonal antibody

RPL27A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16002-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ribosomal protein L27a, Large ribosomal subunit protein uL15, 60S ribosomal protein L27a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16002-1-AP targets RPL27A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL27A fusion protein Ag8596 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L27a
Calculated molecular weight 148 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC005326
Gene Symbol RPL27A
Gene ID (NCBI) 6157
RRIDAB_2878206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL27A antibody 16002-1-APDownload protocol
IHC protocol for RPL27A antibody 16002-1-APDownload protocol
IF protocol for RPL27A antibody 16002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
View Article
humanWB

Bioengineered

tRF-19-W4PU732S promotes breast cancer cell malignant activity by targeting inhibition of RPL27A (ribosomal protein-L27A).

Authors - Zhengxiang Zhang
  • KD Validated
View Article