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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL29 Recombinant antibody (83377-1-RR)

    WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL29 Recombinant antibody (83377-1-RR)

    IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL29 Recombinant antibody (83377-1-RR)

    IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RPL29 Recombinant antibody (83377-1-RR)

    FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83377-1-RR

    Affinity and Kinetic Characterization of 83377-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83377-1-RR against Human RPL29 were performed. The affinity constant is 0.254 nM.

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RPL29 Recombinant antibody, PBS Only

RPL29 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83377-1-PBS
Clone No.240062F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83377-1-PBS targets RPL29 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL29 fusion protein Ag34100 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L29
Calculated molecular weight 159 aa, 18 kDa
GenBank accession numberBC008926
Gene Symbol RPL29
Gene ID (NCBI) 6159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP47914
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.