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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL29 Recombinant antibody

RPL29 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240062A3

Cat no : 83377-2-RR

Synonyms

Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP, 60S ribosomal protein L29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL29 Recombinant antibody (83377-2-RR)

    WB analysis using 83377-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83377-2-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL29 Recombinant antibody (83377-2-RR)

    IF Staining of HepG2 using 83377-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-2-RR, Clone: 240062A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83377-2-RR

    Affinity and Kinetic Characterization of 83377-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83377-2-RR against Human RPL29 were performed. The affinity constant is 1.04 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, U-87 MG cells, C6 cells, NIH/3T3 cells,
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83377-2-RR is RPL29 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL29 fusion protein Ag34100 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L29
Calculated molecular weight 159 aa, 18 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008926
Gene symbol RPL29
Gene ID (NCBI) 6159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL29 antibody 83377-2-RRDownload protocol
IF protocol for RPL29 antibody 83377-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols