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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPL3 Polyclonal antibody (11005-1-AP)

    WB analysis of HeLa using 11005-1-AP (same clone as 11005-1-PBS)

    WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells.. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPL3 Polyclonal antibody (11005-1-AP)

    WB analysis using 11005-1-AP (same clone as 11005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using RPL3 Polyclonal antibody (11005-1-AP)

    WB analysis of human kidney using 11005-1-AP (same clone as 11005-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using RPL3 Polyclonal antibody (11005-1-AP)

    IP experiment of mouse kidney using 11005-1-AP (same clone as 11005-1-PBS)

    IP result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL3 Polyclonal antibody (11005-1-AP)

    IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL3 Polyclonal antibody (11005-1-AP)

    IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL3 Polyclonal antibody (11005-1-AP)

    IF Staining of HepG2 using 11005-1-AP (same clone as 11005-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RPL3 Polyclonal antibody, PBS Only

RPL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11005-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11005-1-PBS targets RPL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL3 fusion protein Ag1436 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L3
Calculated molecular weight 46/27 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012786
Gene Symbol RPL3
Gene ID (NCBI) 6122
RRIDAB_2181760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP39023
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.