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  • Featured Product
  • KD/KO Validated

RPL3 Polyclonal antibody, PBS Only

RPL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11005-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPL3 Polyclonal antibody (11005-1-AP)

    WB analysis of HeLa using 11005-1-AP (same clone as 11005-1-PBS)

    WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells.. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPL3 Polyclonal antibody (11005-1-AP)

    WB analysis using 11005-1-AP (same clone as 11005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using RPL3 Polyclonal antibody (11005-1-AP)

    WB analysis of human kidney using 11005-1-AP (same clone as 11005-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using RPL3 Polyclonal antibody (11005-1-AP)

    IP experiment of mouse kidney using 11005-1-AP (same clone as 11005-1-PBS)

    IP result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL3 Polyclonal antibody (11005-1-AP)

    IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RPL3 Polyclonal antibody (11005-1-AP)

    IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL3 Polyclonal antibody (11005-1-AP)

    IF Staining of HepG2 using 11005-1-AP (same clone as 11005-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11005-1-PBS targets RPL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL3 fusion protein Ag1436 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L3
Calculated molecular weight 46/27 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012786
Gene symbol RPL3
Gene ID (NCBI) 6122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.