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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL3 Recombinant antibody, PBS Only

RPL3 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3P18

Cat no : 82815-1-PBS

Synonyms

ribosomal protein L3, OK/SW-cl.32, Large ribosomal subunit protein uL3, HIV-1 TAR RNA-binding protein B, 60S ribosomal protein L3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using RPL3 Recombinant antibody (82815-1-RR)

    IHC staining of human placenta using 82815-1-RR (same clone as 82815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82815-1-RR (RPL3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82815-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL3 Recombinant antibody (82815-1-RR)

    IF Staining of HepG2 using 82815-1-RR (same clone as 82815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL3 antibody (82815-1-RR, Clone: 3P18 ) at dilution of 1:240 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82815-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RPL3 Recombinant antibody (82815-1-RR)

    FC experiment of HepG2 using 82815-1-RR (same clone as 82815-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPL3 Recombinant antibody (82815-1-RR, Clone:3P18) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82815-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82815-1-PBS targets RPL3 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL3 fusion protein Ag1436 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L3
Calculated molecular weight 46/27 kDa
GenBank accession numberBC012786
Gene symbol RPL3
Gene ID (NCBI) 6122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.